Table 3.
Biodistribution of Ad-NK4 in mice injected into the pleural cavity
| Tissues | Copy numbers of Ad-NK4 per 25 ng of total genomic DNA | ||
|---|---|---|---|
| Day 1 | Day 7 | Day 14 | |
| Brain | 3.9 × 105 ± 1.7 × 105 | 3.4 × 103 ± 1.7 × 103 | 2.3 × 102 ± 1.0 × 102 |
| Lung | 3.1 × 108 ± 2.4 × 108 | 1.3 × 105 ± 3.9 × 104 | 7.5 × 103 ± 3.9 × 103 |
| Stomach | 1.5 × 106 ± 1.1 × 106 | 1.9 × 103 ± 4.3 × 102 | 3.4 × 102 ± 1.4 × 102 |
| Spleen | 6.4 × 105 ± 5.2 × 105 | 4.2 × 104 ± 1.6 × 104 | 2.6 × 103 ± 1.5 × 103 |
| Kidney | 1.3 × 104 ± 4.3 × 103 | 2.0 × 103 ± 6.3 × 102 | 2.1 × 103 ± 9.6 × 102 |
| Liver | 3.2 × 104 ± 1.9 × 104 | 1.9 × 104 ± 8.2 × 103 | 1.2 × 104 ± 1.1 × 104 |
| Small intestine | 1.1 × 104 ± 3.2 × 103 | 3.9 × 102 ± 2.0 × 102 | 6.5 × 102 ± 3.7 × 102 |
| Colon | 1.3 × 104 ± 7.1 × 103 | 9.8 × 102 ± 8.9 × 102 | 1.0 × 102 ± 35 |
| Testis | 4.5 × 103 ± 2.1 × 103 | 2.3 × 102 ± 94 | 45 ± 24 |
| Blood | 1.2 × 104 ± 4.6 × 103 | 2.4 × 103 ± 1.5 × 103 | 2.0 × 102 ± 92 |
Data show average and SE values (n = 6). DNA from respective tissues was amplified to detect a Ad E4 region with the real time polymerase chain reactions as follows, a forward primer, 5′-CACCACCTCCCGGTACCATA-3′, a reverse primer, 5′-CCGCACCTGGTTTTGCTT-3′, and 40 cycles at 62°C for annealing. Ad reference material (VR-1516, ATCC, Manassas, VA, USA) was used as a control.