Table 2. Quantitative analysis of microglial morphology in distinct brain regions in 3.5-months old Syn2-/- mice.
Data are presented as relative percentage (mean ± SEM) of three different microglial phenotypes (Ram-ramified, Inter-intermediate, R/A-round/amoeboid) in motor, somatosensory, and entorhinal cortex; granule cell layer (GCL), molecular layer (ML), and dentate hilus of hippocampus; ventrobasal (VB) and mediodorsal (MD) thalamic nuclei in sub-cortex. n = 10 WT and 7 Syn2-/- mice. Ctx = cortex, HPC = hippocampus, SC = sub-cortex.
| Area | Ram | Inter | R/A | ||||
|---|---|---|---|---|---|---|---|
| WT | Syn2-/- | WT | Syn2-/- | WT | Syn2-/- | ||
| Ctx | Motor | 60.4 ± 4.2 | 51.8 ± 5.2 | 39.6 ± 4.2 | 47.9 ± 5.1 | 0.0 ± 0.0 | 0.4 ± 0.2 |
| Somatosensory | 64.4 ± 4.5 | 59.0 ± 5.6 | 35.6 ± 4.5 | 40.9 ± 5.6 | 0.0 ± 0.0 | 0.0 ± 0.0 | |
| Entorhinal * | 58.7 ± 5.2 | 46.8 ± 6.1 | 41.3 ± 5.2 | 53.1 ± 6.0 | 0.0 ± 0.0 | 0.1 ± 0.1 | |
| HPC | GCL | 55.3 ± 2.5 | 50.5 ± 5.3 | 43.5 ± 2.5 | 47.4 ± 5.0 | 1.2 ± 0.3 | 2.1 ± 1.0 |
| ML * | 69.2 ± 2.7 | 59.4 ± 4.3 | 30.8 ± 2.7 | 40.6 ± 4.3 | 0.0 ± 0.0 | 0.0 ± 0.0 | |
| Hilus * | 58.3 ± 2.7 | 50.5 ± 4.1 | 41.5 ± 2.7 | 49.5 ± 4.1 | 0.0 ± 0.0 | 0.0 ± 0.0 | |
| SC | VB thalamus * | 63.0 ± 5.5 | 45.0 ± 7.3 | 37.0 ± 5.5 | 55.0 ± 7.3 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| MD thalamus | 61.1 ± 5.4 | 51.2 ± 7.5 | 38.9 ± 5.4 | 48.8 ± 7.5 | 0.0 ± 0.0 | 0.0 ± 0.0 | |
*, p ≤ 0.05 representing significant interaction between genotype and morphology by 2-way ANOVA.