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. 2015 Jul 15;10(7):e0130796. doi: 10.1371/journal.pone.0130796

Fig 2. Activities for selected approved drugs versus specified assay modules.

Fig 2

A response of 0% is equivalent to DMSO control, while a 100% response corresponds to the appropriate positive control for the assay (methods). A. The activity of the multikinase inhibitor dasatinib within the Wnt potentiation module. Included are the outcomes in a multilineage potential C2C12 cell line as judged by β-catenin translocation (□) and alkaline phosphatase activity (▲); selectivity of the Wnt response vs. cell cycle effects is assessed by comparison to % inhibition of cell count (■) and % stimulation of 4N DNA content (●) as determined via propidium iodide staining in HeLa cells. B. Inhibition of cellular ATP by the MEK inhibitor PD-184352 in HCT116 cells alone (●) or following pretreatment with a GSK3β inhibitor (▲). C. Inhibition of cellular ATP by the proteasome inhibitor bortezomib in the KRAS assay modules including Colo320 (-●-), DLD-1 (■), HCT116 (▲), HT-29 (▼), RKO (♦), SNU-C1 (●), and SW480 (■). D. Inhibition of cellular ATP by simvastatin, cerivastatin and fluvastatin in the KRAS assay modules including the KRAS-mutant lines SW480 (●), HCT116 (▲) and DLD-1 (+) vs. the KRAS wild type lines HT-29 (◊), RHO (○), SNU-C1 (□) and Colo320 (Δ).