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. 2015 Jul 15;10(7):e0133074. doi: 10.1371/journal.pone.0133074

Fig 5. ETS1 and SP1 suppression are essential for miR-324-5p-inhibited cell migration, invasion and ECM degradation in HCC.

Fig 5

A. The expression levels of ETS1 or SP1 in miR-324-5p-inhibitor transfected HCC cells that were transfected with ETS1 or SP1-siRNA, as measured by western blotting; α-Tubulin served as the loading control. B. Quantification of indicated invading cells in a Matrigel-coated Transwell assay. C. Real-time PCR analysis of MMP2 and MMP9 expression in indicated cells. GAPDH served as control. D. The activity of MMP2 and MMP9 in indicated cells determined by ELISA assay. Each bar represents the mean ± SD of three independent experiments. * P <0.05.