Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 15;10(7):e0131070. doi: 10.1371/journal.pone.0131070

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

Fig 4 — Exponentially growing wild type (MSY-WT2) and yih1Δ (MSY-Y2) cells were arrested in G1 with α-factor and released into fresh SD media. Samples were collected at the indicated time intervals. (A) WCEs of these cultures and of an asynchronous culture (AS) were subjected to immunoblot analysis, using antibodies against eIF2α phosphorylated on Ser-51 (eIF2α-P) and against total eIF2α. (B) Immunoblot signals from three independent blots as shown in (A) were quantified using the NIH Image J software, the ratios between eIF2α-P and eIF2α were determined and the results were normalized using the value of the ratio of the asynchronous cells (AS); values represent means ± S.E.. (C) Representative histograms. Flow cytometry analysis of DNA content of the samples used in (A). Blue, yellow and pink shaded boxes indicate the estimated G1, S and G2/M cell cycle stages, respectively.