Fig 1. n-3 PUFA-enriched diet increases hepatic Tregs.

Hepatic mononuclear cells (HMNCs) and splenocytes were isolated from mice fed with normal diet (ND) and different concentrations of n-3 PUFA-enriched diet (L-PUFA and H-PUFA) for 12 weeks. Tregs were identified by CD4, CD25, and Foxp3 fluorescent staining (n = 10 each group). A) Representative dot plots of CD25⁺Foxp3⁺Tregs in the right upper quadrant (gated from CD4⁺ cells). B) Mean (±SD) percentage of hepatic and splenic Tregs among CD4⁺ cells from ND, L-PUFA and H-PUFA fed mice. C) Mean (±SD) percentage of hepatic Tregs among CD4⁺ cells from mice fed with n-3 PUFA-enriched diet for different periods (n = 5 each group). *p<0.05 vs ND mice