Fig 3. Effect of n-3 PUFA on the proliferation of Tregs in vivo.

To assess the proliferation of Tregs in vivo, mice consuming ND diet or low concentration of n-3 PUFA (L-PUFA) diet for 4–12 weeks were administered BrdU (0.8mg/ml) in drinking water for 7 consecutive days before sacrifice. HMNCs and splenocytes were isolated as above and fluorescent stained. A) Representative dot plots of BrdU expression in Foxp3⁺ cells in the right upper quadrant (gated from CD4⁺CD25⁺ cells). B) Mean (±SD) percentage of BrdU⁺ Tregs in liver and spleen (n = 5 each group). C) Mean (±SD) percentage of hepatic BrdU⁺ Tregs from mice fed with L-PUFA-enriched diet for different periods (n = 5 each group). *p<0.05 vs ND mice