Table 1.

Tight junction targeting domains of Cdc42/RhoA GEFs and GAPs.

	Targeting sequence/domain	Junctional component	Function	Reference(s)
GEF
Tuba	C-terminal region containing SH3 domains, plus additional, undetermined aa residues	TJ ZO-1 (CPAP), Tricellulin/MARVELD2 (ITMP)	TJ configuration, tension via activation of Cdc42/N-WASP Effector, promoting actin polymerization	Otani et al. (2006), Oda et al. (2014)
DBL3	N-terminal Sec 14-like region, pleckstrin homology (PH) domain	Ezrin (TJAMP), membrane phospholipids?	TJ positioning via activation of Cdc42/Par6-aPKC Effector complex	Ognibene et al. (2011), Zihni et al. (2014b)
ECT2	N-terminal regulatory domain (cyclin b6, BRCT1/2, S), C-terminal (DH, PH) region	TJ Par6 (CPAP)	TJ assembly and/or maintenance via activation of the Cdc42/Par6-aPKC-Par3 effector complex	Liu et al. (2004, 2006)
p114 RhoGEF	N-terminal pleckstrin homology (PH) domain, C-terminal PDZ (PSD-95/Dlg/ZO-1) binding motif (PBM)	TJ Cingulin (CPAP), TJ PALS 1-associated tight junction Protein (PATJ) (CPAP)	TJ assembly and maturation via RhoA/ROCKII-dependent MLC phosphorylation	Terry et al. (2011), Nakajima and Tanoue (2012)
ARHGEF11	C-terminal region	TJ ZO-1 (CPAP)	TJ assembly and maturation via RhoA-dependent MLC phosphorylation	Itoh et al. (2012)
GEFH1	PH domain	TJ Cingulin/JACOB (CPAP)	Inhibition of Rho A dependent proliferation following TJ assembly	Aijaz et al. (2005)
GAP
SH3BP1	N-terminal Bar domain C-terminal SH3 domain	TJ Jacob (paracingulin) (CPAP) CD2AP	TJ assembly via regulation of Cdc42 at nascent cell-cell contacts, inactivation of actin Polymerization and Actin capping	Elbediwy et al. (2012)
Rich1	N-terminal Bar domain	TJ angiomoitin (AMOT) (CPAP)	TJ maturation/integrity via regulation of Cdc42, linking TJ to intracellular protein trafficking	Wells et al. (2006)

CPAP, cytoplasmic plaque adapter protein; ITMP, integral transmembrane protein; TJAMP, tight junction apical margin protein.