Fig. 1.
Illustration of the motion artifacts removal by computational image processing. Left-hand side panels illustrate raw data, right-hand side panels – data extracted from the motion corrected sequence. From top to bottom: maximum intensity projections of image sequence containing two contraction–relaxation cycles; pseudo line-scan images obtained by re-slicing along the line in top panel; and superposition of normalised [Ca2+]i traces from a single cell indicated by a rectangle in the middle panel (solid line) and from the entire field (dashed line). Note almost complete removal of motion artifacts after image processing on the right. White scale-bars in the middle panel correspond to 120 s.