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. 2015 Jul 11;17(2):273–287. doi: 10.22074/cellj.2016.3730

Fig.5.

Fig.5

Effect of VEGF-siRNA and/or doxorubicin treatment on apoptosis in Hep3B cells. A. Cell apoptosis was detected using annexin V-FITC/ propidium iodide (PI) staining and flow cytometry analysis. Fluorescence intensity of annexin V/FITC is plotted on the x-axis, and PI is plotted on the y-axis. Cells in the lower left (LL) quadrant represent survivals, lower right (LR) quadrant represent early apoptosis, the upper right (UR) quadrant represent necrosis or post-apoptotic and the upper left (UL) quadrant represent detection of error allowed and B. The percentage of apoptotic cells examined by annexin V-FITC/PI staining and flow cytometry analysis. Each bar represents the mean value ± standard deviation (SD) intensity of fluorescent positive cells during early apoptotic events of triplicate experiments. *; P<0.05, **; P<0.01 compared to control cell group (or medium), #; P<0.05 compared to VEGF-siRNA treated cell group, Dox 0; 0 μg/ml (or medium) doxorubicin, Dox 1; 1 μg/ml doxorubicin, Dox 2; 2 μg/ml doxorubicin, Dox 4; 4 μg/ml doxorubicin, VEGF-siRNA; Vascular endothelial growth factor targeted small-interfering RNA and FITC; Fluorescein isothiocyanate.