Fig. 2.

Polarization of immune response is elicited by recombinant spores as assessed by analysis of profiles of cytokines secreted by sensitized splenocytes. Splenocytes were isolated from mice orally immunized with the wild-type 168 or recombinant BKH108 (UreB) and BKH121 (IL-2) spores as described in “Methods” section. Levels of cytokines (pg/ml) indicated in each graph were measures by flow cytometry using the cytometric bead array. Graphs are arranged in groups of cytokines characteristic for polarizations of the immune response indicated with Th1, Th2, or Th17. The presented results are averages of cytokines levels secreted by splenocytes isolated from groups of eight animals. Error bars indicate standard deviation. Statistical significance versus 168 assessed by Student’s t test *p < 0.05, **p < 0.01, ***p < 0.001