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. 2015 Jul;99:172–177. doi: 10.1016/j.steroids.2015.02.021

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — Oxysterols and cholestenoic acids in mouse CSF. (A) RIC for m/z 534.4054 showing 24S-HC, 25-HC, 26-HC and B-ring oxidised sterols. (B) RIC for m/z 550.4003 showing 7α,25-diHC/7α,25-diHCO, 7α,24-diHC/7α,24-diHCO, and 7α,26-diHC/7α,26-diHCO. (C) RIC for m/z 548.3847 showing 3β-HCA. (D) RIC for m/z 564.3796 showing 3β,7α-diHCA/7αH,3O-CA. Note that sterols derivatised with the GP reagent form a mixture of syn and anti isomers which may or may not be resolved e.g. 3β,7α-diHCA/7αH,3O-CA elute as syn and anti isomers at 5.77 and 6.61 min, and the second isomer of 7α,24-diHC/7α,24-diHCO elutes at 6.61 min.