Fig. 3.

GFP expression upon HFR UV-B in pmARI12:ARI12-GFP lines are controlled by COP1. 25 day old plants were exposed to broad band HFR UV-B (4 μmol m⁻² s⁻¹) for 90 min (10:30–12:00). Leaves were harvested before (−UV) and at different time points after UV-B exposure. (A) ARI12-GFP expression was quantified with real-time PCR and normalized to the reference gene UBQ5. (B) Fold ARI12-GFP expression was compared with samples before UV-B exposure. (C) Western blots were probed with anti-GFP and anti-actin antibodies and (D) quantified and normalized to the actin loading control. (E) Detection of ARI12-GFP K8 in leaves of 25 day old plants around 6 h after a 90 min UV-B treatment. Pictures were taken on a CLSM.