Table 2.
Primary antibodies and antigen retrieval
| Primary antibody | Antibody type | Target | Source | Staining |
|---|---|---|---|---|
| CA II | Sheep (pAB) | Carbonic anhydrase II | PC076; BindingSite | 1:1500; E |
| CD68 | Mouse (mAB) | Cluster of differentiation 68 | M0814; Dako | 1:100; E |
| E06 | Mouse (mAB) | Oxidized phospholipids | [34] | 10 µg/ml; none |
| GFAP | Mouse (mAB) | Glial fibrillary acid protein | 0410080; ThermoSc | 1:200; E |
| HLA-DP, DQ, DR | Mouse (mAB) | Human leukocyte antigen | M0775; Dako | 1:100; E |
| HO-1 | Rabbit (pAB) | Heme oxygenase-1 | ab13243; Abcam | 1:2000; CSA; C |
| KEAP1 | Mouse (mAb) | Kelch-like ECH-associated protein 1 | NBP2-03319, Novus Biologicals | 1:250; E |
| MAG | Mouse (mAB) | Myelin-associated glycoprotein | ab89780; Abcam | 1:1000; E |
| MAP 2 | Mouse (mAB) | Microtubule-associated protein 2 | M4403; Sigma | 1:100; E |
| Nrf2 | Rabbit (mAB) | Nuclear factor (erythroid-derived 2)-like 2 | ab62352; Abcam | 1:100; E |
| Nrf2 | Rabbit (pAB) | Nuclear factor (erythroid-derived 2)-like 2 | C-20; Santa Cruz | 1:500; C |
| p22phox | Rabbit (pAB) | NADPH oxidase | Sc-20781; Santa Cruz | 1:100; C |
| PLP | Mouse (mAB) | Proteolipid protein | MCA8394; AbD Serotec | 1:1000; E |
| Tppp/p25 | Rat (pAB) | Tubulin polymerization-promoting protein | [23] | 1:100; E |
mAB monoclonal antibody, pAB polyclonal antibody, C citrate buffer pH 6, E ethylenediaminetetraacetic acid buffer pH 9.0