Fig. 5.

Comparison of Hmgcr mRNA and HMGCR protein levels following 25-HC or IFN-$\gamma$ treatment in wild-type and Ch25h^−/− BMDMs. (a) Western blot analysis of HMGCR with 2.5 μM of 25-HC or 5 ng/mL of IFN-$\gamma$ treatment in Medium C at 4-h and 9-h time points in wild-type BMDMs. (b) Intensity values of HMGCR to $\beta$-actin. Bars present the mean ± SEM (n = 3). (c) Western blot analysis of HMGCR with 2.5 μM of 25-HC or 5 ng/mL of IFN-$\gamma$ treatment in Medium C at 4-h and 9-h time points in Ch25h^−/− BMDMs. (d) Intensity values of HMGCR to $\beta$-actin. Bars present the mean ± SEM (n = 3). (e) HMGCR transcript abundance in wild-type and Ch25h^−/− BMDMs with 2.5 μM of 25-HC or 5 ng/mL of IFN-$\gamma$ treatment for 24 h in Medium A. The 25-HC-treated groups were normalised by the Vehicle and the IFN-$\gamma$-treated groups were normalised by the control treated samples, respectively. Bars present the mean ± SEM (n = 9). ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, determined with an unpaired Student’s t test.