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. 2015 Jul 16;6:199. doi: 10.3389/fphys.2015.00199

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Copyright © 2015 Woodward, Previs, Mader and Debold.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Purification with HIC column enhances V_actin. Further purification of the myosin using an HIC column nearly doubled V_actin from both Control tissue and tissue exposed to ischemia (IR2) and 2 h of reperfusion (p < 0.001). However, there was no difference between conditions with either the isolated myosin or the HIC purified myosin. The data represent the average actin filament velocities from 106 to 30 s videos from Control and 19 from IR2, as well as five videos each for HIC purified Control and IR2 purified samples. ^* Indicates significance at p < 0.05.