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. 2015 Jul 16;6:199. doi: 10.3389/fphys.2015.00199

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Copyright © 2015 Woodward, Previs, Mader and Debold.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of ischemia/reperfusion on native thin filament velocities (V_NTF). Means ± SEM for unregulated (no Tn/Tm) actin filament velocities in the in vitro motility assay using myosin isolated from the corresponding condition. The myosin used was isolated from control, ischemia/2 h of reperfusion (IR2) and Ischemia without resuscitation (IDNR). Data analyzed using a non-parametric Kruskal–Wallis ANOVA and indicated that IDNR was significantly greater than control velocity ^*, (p < 0.05). The data represent the average actin filament velocities from 20 to 30 s videos from Control, 19 from IR2 and 16 from IDNR.