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. 2015 Jul 16;6:719. doi: 10.3389/fmicb.2015.00719

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Copyright © 2015 Bonis and Gralnick.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fe(II) oxidation under respiratory inhibition. Representative set of Marinobacter strain JG233 in tubes containing FeS as the Fe(II) source and MIM +0.15% low melt agarose as the medium overlay. Results were consistent regardless of incubation time prior to respiratory inhibition, as long as inhibitor was added prior to rust-colored band formation. Tubes (A) and (B) contain sodium azide to 2 mM both abiotic and with Marinobacter strain JG233, respectively. N,N-dicyclohexylcarbodiimide solubilized in 200 proof ethanol was added to a final concentration of 20 μM to an abiotic control (C) and a tube with Marinobacter strain JG233 (D). The tubes containing respiratory inhibitors exhibited rust-colored bands dissimilarly to uninhibited Marinobacter strain JG233 (E) and are located in similar positions as the abiotic control (F), indicating Fe(II) oxidation by Marinobacter strain JG233 required actively metabolizing cells.