Table 1.
Bacterial strains, vectors, and primers.
| Strain or vector | Relevant trait(s) | Source | |
|---|---|---|---|
| Strains | |||
| Marinobacter subterrani | |||
| JG233 | Wild Type | This study | |
| JG2729 | ΔflaBG | This study | |
| JG3126 | Wild Type with pBBR1MCS-2 | This study | |
| JG2756 | ΔflaBG with pBBR1MCS-2 containing flaBG | This study | |
| JG2791 | JG233 with pBBR1MCS-2 containing gfpmut3 | This study | |
| Escherichia coli | |||
| UQ950 | DH5α vector construction host | Saltikov and Newman (2003) | |
| WM3064 | DAP auxotroph donor strain for conjugation | Saltikov and Newman (2003) | |
| Vectors | |||
| ptpSMV3 | Deletion vector, Kmr, sacB | Saltikov and Newman (2003) | |
| ptpSMV3ΔflaBG | Deletion construct for flaBG | This study | |
| ptpBBR1MCS-2 | Broad range cloning vector, Kmr | Kovach et al. (1995) | |
| ptpBBR1MCS-2::flaBG | ΔflaBG complementation vector | This study | |
| ptpBBR1MCS-2::gfpmut3 | gfp containing vector | Laboratory stock | |
| ptpMiniHimar RB1 | Transposon-carrying plasmid | Bouhenni et al. (2005) | |
| Primers | |||
| Flagellin UF | catgGGATCCTTCTTCCTGTTTGGGACCGAC | This study | |
| Flagellin UR | catgCTTAAGGGCTAATGCCCCTCCAGTATC | This study | |
| Flagellin DF | cagtCTTAAGCAGTAAACTCAGAACGCCC | This study | |
| Flagellin DR | cagtACTAGTGTGCCGGTTTCCTCGGAG | This study | |
| Flagellin CompF | cagtAAGCTTGATACTGGAGGGGCATTAGCC | This study | |
| Flagellin CompR | cagtACTAGTGGGCGTTCTGAGTTTACTG | This study | |
| 27F | AGA GTT TGA TCM TGG CTC AG | Wilson et al. (1990) | |
| M13F | GTA AAA CGA CGG CCA GT | Universal Primer List, n.d. | |
| ptM13R | CAG GAA ACA GCT ATG AC | Universal Primer List, n.d. | |
Restriction site indicated in bold.