Reagent	Type	Manufacturer	Catalog #	Comments
60 mm tissue culture dishes	Labware	Sigma–Aldrich	CLS430166
PBS, without MgCl2 and CaCl2	Buffer	Sigma–Aldrich	D8537	Original not specified
RIPA lysis buffer	Buffer	Specific brand information will be left up to the discretion of the replicating lab and recorded later
Cell scraper	Labware
Refrigerated centrifuge	Equipment
Bradford assay	Reporter assay
Molecular weight marker	Western materials
6× SDS-PAGE sample buffer	Buffer	Specific brand information used to make these reagents will be left up to the discretion of the replication lab and recorded later
SDS-PAGE gel	Western materials
Tris-glycine SDS-PAGE running buffer	Buffer
Electrotransfer buffer	Buffer
Ponceau S stain	Stain
TBS buffer	Buffer
PVDF membrane	Western materials	Specific brand information will be left up to the discretion of the replicating lab and recorded later
Tween-20	Chemical
Non-fat dry milk	Western materials
ECL chemiluminescent reagent	Western materials
Mouse anti-Cav1 (clone 2297)	Antibodies	BD Biosciences	610406	Original clone/catalog# unspecified
Mouse anti-α-smooth muscle actin (clone 1A4)	Antibodies	Sigma–Aldrich	A5228
Mouse anti-γ-tubulin (clone GTU-88)	Antibodies	Sigma–Aldrich	T6557	Original clone/catalog # unspecified
Goat anti-mouse-HRP	Antibodies	Life Sciences	32,430