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. 2015 Jul 16;5:11944. doi: 10.1038/srep11944

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a) Non-canonical polypeptide cargo (green star) ligated to the C-terminus of LF_N (pdb: 1J7N) to translocate through PA pore (pdb: 3J9C) b) Sortase A-mediated ligation of LF_N-DTA-LPSTGG and G₅-cargo to form constructs are comprised of LF_N-DTA and non-canonical polypeptide cargo (LDn).