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. 2015 Jul 16;5:11944. doi: 10.1038/srep11944

Figure 4. Translocation of small molecules.

Figure 4

a) Small molecules doxorubicin, docetaxel, and monomethyl auristatin F (MMAF) were conjugated at the cysteine residue of G5-LRRLRAC then ligated to LFN-DTA to form LDn9–11. b) CHO-K1 cells were treated with ten-fold serial dilutions of LDn9–11 and LFN-DTA in the presence of 20 nM PA for 30 minutes. The same experimental conditions were used as previously described. c) CHO-K1 cells were treated with 100 nM LDn9–11 in the presence of 20 nM PA for 12 hours then digitonin extracted and run on western blot, as previously described. Cropped blots are used in western blot data (c).