Figure 5. Translocation of C-terminally biotinylated cargo.

a) Non-canonical peptides 1–6 were modified to contain biotin on a C-terminal lysine residue (1-bio to 6-bio) then ligated to LF_N-DTA to form LDn1-bio to 6-bio b) Peptides containing the small molecules, doxorubicin and monomethyl auristatin F (MMAF) were modified to contain biotin on a C-terminal lysine residue (9-bio and 11-bio, respectively) then ligated to LF_N-DTA to form LDn9-bio and LDn11-bio c) CHO-K1 cells were treated with 100 nM LDn2, LDn9, LDn11, LDn1-bio to LDn6-bio, LDn9-bio, and LDn11-bio in the presence of 20 nM PA for 12 hours then digitonin extracted and run on western blot, as previously described. Cropped blots are used in western blot data (c).