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. 2015 Apr 13;59(2):2477. doi: 10.4081/ejh.2015.2477

Table 1.

Lectins and corresponding source, carbohydrate binding specificity, inhibitory carbohydrate, used concentration, and fluorescent conjugate (labelling).

Lectin
Helix pomatia agglutinin Wheat germ agglutinin
Acronym HPA WGA
Source Helix pomatia Triticum vulgaris
Binding specificity GalNAc47 GlcNAc48>>Neu5Ac49
Inhibitory carbohydrate GalNAc* (GlcNAc)3>(GlcNAc)2>GlcNAc >>Neu5Ac>>GalNAc*
Concentration (mg/mL) 0.005 0.025
Fluorescent conjugate TRITC FITC

Gal, galactose; GalNAc, N-acetylgalactosamine; GlcNAc, N-acetylglucosamine; Neu5Ac, N-acetylneuraminic acid (sialic acid); TRITC, tetramethylrhodamine; FITC, fluorescein isothiocyanate;

*according to the original data sheet provided by manufacturer.