Table 2.
Lectin | Stain | |||||||
---|---|---|---|---|---|---|---|---|
WGA | HPA | PAS | PAS-AB | pH 2.5 | ||||
Esophagus | ||||||||
Mucous surface cells | +++ | S | +++ | S | +++ | +++ | (m) | N |
Lumen mucus | ++ | - | +++ | +++ | (m) | N | ||
Stomach | ||||||||
Mucous surface cells | +++ | S | - | +++ | +++ | (m) | N | |
Chief cells | +++ | S | +++ | C | - | - | ||
Neck cells | ++ | S | - | + | + | (p) | M | |
Parietal cells | ++ | S | + | S | ++ | ++ | (p) | M |
Lumen mucus | - | ++ | +++ | +++ | (m/p) | N/M | ||
Duodenum | ||||||||
Enterocytes | + | S | + | S | + | + | (m) | N |
Goblet cells | +++ | C | + | S | +++ | +++ | (p) | M |
Jejunum-ileum | ||||||||
Enterocytes | - | + | S | + | + | (m) | N | |
Goblet cells | +++ | C | + | C | +++ | +++ | (p) | M |
Lumen mucus | +++ | +++ | +++ | +++ | (p) | M | ||
Ileum-colon | ||||||||
Enterocytes | +++ | S | ++ | S | + | + | (m) | N |
Goblet cells | +++ | C | + | C | +++ | +++ | (p/b) | M/A |
Lumen mucus | +++ | + | +++ | +++ | (p/b) | M/A | ||
Colon-rectum | ||||||||
Enterocytes | + | S | - | +++ | +++ | (m/p) | N/M | |
Goblet cells base of crypts | + | S | + | S | +++ | +++ | (b) | A |
Goblet cells top of crypts | + | S | + | S | +++ | +++ | (p/m) | M/N |
Lumen mucus | +++ | - | +++ | +++ | (b-p-m)* | A-M-N* |
+++, strong positive; ++, positive; +, weak positive; -, negative; S, surface; C, cytoplasm; m, magenta; p, purple; b, blue; N, neutral carbohydrates; M, mixed carbohydrates; A, acid carbohydrates;
*, from the base to top of the crypt.