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. 2015 Jul 16;5:12126. doi: 10.1038/srep12126

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Testes of adult mice were subjected to squash preparation and then immunofluorescence staining. (A,B) The round spermatids were immunostained with anti-CaMKIV antibody (green). (C) Immunofluorescence staining of MVH (green). The parallel bright-field images demonstrate the location of the chromatoid bodies, which are indicated by arrows. Alexa Fluor 488 anti-rabbit IgG was used as a secondary antibody, and nuclei were stained blue with Hoechst 33342 dye. Scale bar: 5 μm.