Figure 2. Interaction of CaMKIV with MVH and MIWI.
(A) In HEK293T cells, plasmids of FLAG-CaMKIV-FL (the full length of CaMKIV) or FLAG-CaMKIV-CA (constitutively active form of CaMKIV) were co-transfected with GFP-MVH; (B) plasmids of FLAG-CaMKIV-FL or FLAG-CaMKIV-CA were transfected with GFP-MIWI. Immunoprecipitation from the cell lysates was performed with anti-mouse FLAG antibody, and the samples were immunoblotted by anti-mouse GFP antibody to detect MVH or MIWI, and then by anti-mouse FLAG antibody to detect coimmunoprecipitated CaMKIV. (C) Mouse testes were immunoprecipitated by anti-rabbit MIWI antibody, and the samples were immunoblotted by anti-rabbit MIWI antibody, then by anti-mouse CaMKIV antibody to detect coimmunoprecipitated CaMKIV. Anti-rabbit GFP antibody was used as a negative control.