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. 2015 Jul 16;5:12126. doi: 10.1038/srep12126

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Copyright © 2015, Macmillan Publishers Limited

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HEK293T cells were transfected with the indicated combinations of expression plasmids for FLAG-KIF17b-FL, GFP-CaMKIV-CA, GFP-MVH, and GFP-MIWI. The cell lysates were precipitated by anti-mouse GFP antibody. KIF17b and CaMKIV were immunoblotted by anti-mouse FLAG antibody, whereas MVH and MIWI were immunoblotted by anti-mouse GFP antibody. The total DNA in each group was kept constant using an empty FLAG plasmid.