Fig 3.
Increased length of glutamate domain confers greater retention to three distinct bone grafts after 3 days with vigorous agitation. Bone grafts were coated with DGEA, E2DGEA, E4DGEA, and E7DGEA (FITC-tagged) as described previously. Samples were then washed briefly in TBS, and resuspended in fresh TBS with agitation for 3 days. Peptide release was measured by monitoring the appearance of solution fluorescence. As the length of the glutamate domain increased, more peptide was retained on the graft surface despite agitation for 3 days. Significant difference (P < .05) between samples was denoted as follows: relative to DGEA = a, E2DGEA = b, E4DGEA = c, E7DGEA = d, and * to all.