Fig. 4.

Inhibition of sulfatide-stimulated FXII autoactivation by rPLTP in the presence of VLDL. a The time course of inhibition of sulfatide-stimulated factor XII autoactivation by rPLTP was determined when factor XII (0.1 μM, final) was pre-incubated with rPLTP (5 μg/mL) in the presence of VLDL (25 μg/ml protein, final) for 15 min at room temperature, and sulfatide vesicles (final 0.5 μg/ml) were added to start autoactivation of factor XII. Then, S2302 was added at various time points and the amidolytic activity was measured at different time points. The rPLTP concentration was 0 (○) or 5 μg/ml (●). b The dose–response for rPLTP inhibition of sulfatide-stimulated factor XII autoactivation by rPLTP was determined as in panel (a) and data for factor XIIa activity at 15 min are shown