Fig. 3.

Functional characterization of lymphoid follicles in the granulomas spleen determined by immunohistochemistry on paraffin-embedded sections of spleen stained with antibody against Ki67. a Naïve (uninfected) mice used as control, b spleen with granulomas, c spleen without granulomas. d serum SEA specific IgG and IgM (1:50 dilutions) measured by ELISA. The groups were mice with granulomatous spleens (n = 7), non-granulomatous spleens (n = 8) and naïve mice (n = 8). Bars indicate means ± standard deviations. Scale = 200 μm