Fig. 1.
The Tks4R43W mutant protein is present in detergent-insoluble pellets. 1a, COS7 cells were transiently transfected with wild type V5-Tks4 or V5-Tks4R43W constructs. After 18 h the cells were lysed, and the lysates were separated into detergent-soluble (S) and insoluble pellet fractions (P) by centrifugation and analyzed by Western blotting for Tks4 (with anti-V5 antibody), Cbl, caveolin-1 and HDAC6, respectively. 1b, Wild type and mutant Tks4 protein levels were determined by densitometry. Error bars represent the standard deviation from three independent experiments. Asterisks indicate the level of significance (one asterisk, P < 0.05; two asterisks, P < 0.01; three asterisks, P < 0.001)