Fig. 4.
Perinuclear Tks4R43W aggregate formation is microtubule and HDAC6-dependent. 4a, COS7 cells were transiently transfected with wild type V5-Tks4 or V5-Tks4R43W constructs. After 4 h, the cells were treated with 1,6 μM nocodazole for 16 h. The cells were fixed and stained for Tks4 (with anti-V5 antibody, red) and α-tubulin (green). 4b, The percentage of aggresomes and punctate structures in cells was quantified by counting 300 cells/sample by using ImageJ software. 4c, COS7 cells were transiently transfected with wild type V5-Tks4 or V5-Tks4R43W constructs. After 4 h, the cells were treated with 1 μM Trichostatin A (TSA) for 16 h. The cells were fixed and stained for Tks4 (with anti-V5 antibody, red) and α-tubulin (green). 4d, The percentage of aggresomes and punctate structures in cells was quantified by counting 300 cells/sample by using ImageJ software. Error bars represent the standard deviation from three independent experiments. Asterisks indicate the level of significance (one asterisk, P < 0.05; two asterisks, P < 0.01; three asterisks, P < 0.001)