Table 2.
qPCR results on original and archived PAHC duplicate oropharangeal swabs from five pools screening potentially positivea
| Pool | Animal ID | Species | Location | Original sample pool Ct | Original sample: individual Ct | Duplicate sample: individual Ct |
|---|---|---|---|---|---|---|
| 2 | U95 | C. brachyotis | Puning Cave | 42.0/ND | 41.2/ND | |
| U96 | Pt. jagori | Puning Cave | ||||
| U97 | Pt. jagori | Puning Cave | ||||
| U98 | Pt. jagori | Puning Cave | ||||
| U99 | Pt. jagori | Puning Cave | ||||
| 3 | R1 | M. australis | Biak na Bato | 43.0/ND | ||
| R2 | M. australis | Biak na Bato | ||||
| R3 | M. australis | Biak na Bato | ||||
| R4 | M. australis | Biak na Bato | 39.3/ND | |||
| R5 | M. australis | Biak na Bato | ||||
| 4 | T67 | M. australis | Biak na Bato | 40.6/41.9b | ||
| T68 | M. australis | Biak na Bato | ||||
| T69 | M. schreibersii c | Biak na Bato | 33.6d | |||
| T70 | M. schreibersii c | Biak na Bato | 37.7d | 39.9/ND | ||
| T71 | M. schreibersii c | Biak na Bato | 32.9d | 40.1/ND | ||
| T72 | M. schreibersii e | Biak na Bato | ||||
| T73 | M. schreibersii e | Biak na Bato | ||||
| T74 | M. schreibersii e | Biak na Bato | 40.5/ND | |||
| T75 | M. schreibersii e | Biak na Bato | ||||
| T76 | M. schreibersii e | Biak na Bato | ||||
| 6 | T56 | Ch. plicata | Biak na Bato | 39.7/40.1b | ||
| T57 | Ch. plicata | Biak na Bato | ||||
| T58 | Ch. plicata | Biak na Bato | ||||
| T59 | Ch. plicata | Biak na Bato | ||||
| T60 | M. schreibersii | Biak na Bato | ||||
| 12 | U21 | M. australis | Biak na Bato | 40.2/ND | 42.2/ND | |
| U22 | M. australis | Biak na Bato | ||||
| U23 | Ch. plicata | Biak na Bato | ||||
| U24 | Ch. plicata | Biak na Bato | ||||
| U25 | Ch. plicata | Biak na Bato |
aAll samples were tested in duplicate. Positive samples were confirmed in triplicate
bPools 4 and 6 had repeatable results on the original pooled sample
cT69, T70 and T71 yielded a product on hemi-nested PCR whose sequence differed by one nucleotide from a pig isolate in Bulacan province
dMean value of the duplicates
eAdditional M. schreibersii samples from a pool which tested negative in the original round