Fig 1.

Effects of YSY01A on 26S proteasomes. A. YSY01A inhibited 26S proteasomes from PC-3M cells. B. IC₅₀ values of YSY01A and PS341 on three catalytic sites. C. PC-3M cells were exposed to YSY01A (12.5-400 nM) for 3 h, expression of β catalytic sites and IOD of these bands were shown. D. Ubiquitinated proteins were displayed after YSY01A (12.5-100 nM) treated PC-3M cells for 48h. Every panel is the representative image of three independent experiments. Columns, mean of three independent experiments; bars, SD; compared with control, * p<0.05, ** p<0.01, *** p<0.001