Figure 2.

(A) The methylation status of the 3OST2 promoter in normal liver cells and in HCC cells with or without 5-Aza-CdR and/or TSA treatment. The 3OST2 promoter was not methylated in normal liver cells and completely methylated (only methylated alleles were present) in HCC cells. This was partially reversed by 5-Aza-CdR or TSA treatment (both unmethylated and methylated alleles were present). When 5-Aza-CdR was combined with TSA, the promoter was completely reversed (only unmethylated alleles were present). (B) Real-time reverse transcription PCR (qRT-PCR) analysis of 3OST2 mRNA expression in HCC cells. Expression was normalized using β-actin as an internal control.