Figure 5.

ChIP-qPCR assays for H3R8me2s and H3R8me2a on the 3OST2 promoter in normal liver cells and HCC cells with or without 5-Aza-CdR and/or TSA treatment. Cross-linked chromatin from HCC cells treated or untreated with 5-Aza-CdR and/or TSA underwent immunoprecipitation using control IgG, anti-UHRF1, anti-H3R8me2s or anti-H3R8me2a antibodies; the immunoprecipitated DNA was analyzed by qPCR using primers specific for 3OST2. Shown is the enrichment, relative to the input obtained in each ChIP samples.