Table 1.
The Methylation-specific PCR primers of ten tumor suppressor genes
| Gene | Sense 5'-3' | Antisense 5'-3' |
|---|---|---|
| 3OST2(M) | CGGTTGTTCGGAGTTTTATC | GTAACGCTACCACGACCACG |
| 3OST2(U) | TGGAGTTTTATTGTTTAGGATT | AAAACTCACATAACACTACCACA |
| ppENK(M) | TGTGGGGAGTTATCGAGC | GCCTTCGCGAAAAAAATCG |
| ppENK(U) | TTGTGTGGGGAGTTATTGAGT | CACCTTCACAAAAAAAATCAATC |
| CHFR(M) | TTTCGTGATTCGTAGGCGAC | GCGATTAACTAACGACGACG |
| CHFR(U) | TTTTGTGATTTGTAGGTGAT | ACAATTAACTAACAACAACA |
| LKB1(M) | ACGAAGTTGATTTTGATCGGGTC | CGATACAAAATCTACGAACCGACG |
| LKB1(U) | GGATGAAGTTGATTTTGATTGGGTT | ACCCAATACAAAATCTACAAACCAACA |
| THBS1(M) | TGCGAGCGTTTTTTTAAATGC | TAAACTCGCAAACCAACTCG |
| THBS1(U) | GTTTGGTTGTTGTTTATTGGTTG | CCTAAACTCACAAACCAACTCA |
| HIC1(M) | TCGGTTTTCGCGTTTTGTTCGT | AACCGAAAACTATCAACCCTCG |
| HIC1(U) | TTGGGTTTGGTTTTTGTGTTTTG | CACCCTAACACCACCCTAAC |
| SLIT2(M) | CGGTTTAGGTTGCGGCGGAGTCGAGGGC | CGCGAAAACCCAACGAACCCGTAACAAAACGCG |
| SLIT2(U) | TGGTTTAGGTTGTGGTGGAGTTGAGGGT | CACAAAAACCCAACAAACCCATAACAAAACACA |
| EDNRB(M) | AAATTGCGGAGCGGTTATC | CCAAATCCGCGACAAACCG |
| EDNRB(U) | GGTTTTGAAATTGTGGAGTGG | TCCCCAAATCCACAACAAAC |
| COX2 (M) | TTAGATACGGCGGCGGCGGC | TCTTTACCCGAACGCTTCCG |
| COX2 (U) | ATAGATTAGATATGGTGGTGGTGGT | CACAATCTTTACCCAAACACTTCCA |
| CLDN-7(M) | GACGTTAGGTTATTTTCGGTC | AAACGCGTTTCTAAACGCCG |
| CLDN-7(U) | TGGGGAAAGGGTGGTGTTG | TTACCCAATTTTAACCACCAC |