Table 2.
Methylation profiling of tumor suppressor genes in primery HCCs and the matched corresponding noncancerous tissues
| Gene | Annealing temperature (℃) | Located chromosome |
Methylated rate in HCCs |
Methylated rate in non-HCCs |
|---|---|---|---|---|
| 3OST2 (M) | 60 | 16p12 | 68.8%* | 37.5% |
| 3OST2 (U) | 60 | |||
| ppENK(M) | 62 | 8q23-q24 | 56.3% | 56.3% |
| ppENK(U) | 62 | |||
| CHFR (M) | 58 | 12q24.33 | 20.8%** | 2.1% |
| CHFR (U) | 53 | |||
| LKB1(M) | 60 | 19p13.3 | 4.2% | 0% |
| LKB1(U) | 60 | |||
| THBS1(M) | 62 | 15q15 | 16.7% | 22.9% |
| THBS1(U) | 62 | |||
| HIC1(M) | 60 | 17p13.3 | 66.7% | 85.4% |
| HIC1 (U) | 60 | |||
| SLIT2 (M) | 68 | 4p15.2 | 97.9% | 95.8% |
| SLIT2 (U) | 59 | |||
| EDNRB(M) | 58 | 13q22 | 93.8% | 81.3% |
| EDNRB(U) | 58 | |||
| COX2 (M) | 60 | 1q25.2- q25.3 | 25%** | 4.2% |
| COX2 (U) | 60 | |||
| CLDN7 (M) | 56 | 17p13 | 10.4% | 14.6% |
| CLDN7 (U) | 56 |
HCC, Hepatocellular carcinoma.
P value is relative to non-HCCs ( χ2 tests). *P < 0.05; ** P < 0.01.