Fig 3.

RGS17 promotes migration, invasion, and proliferation of breast cancer cells (ZR-75-30). (A) Western blot analysis of RGS17 in cells stably transfected with specific shRNA or scramble sequence shRNA (Control). (B) Photographs at 0h and 24h after wounding were made in control and RGS17 knockdown cells as results of a wound-healing assay. The mean ± SD of percentage of migration distances obtained from three parallel experiments. ***p<0.001. (C) Detection of cell proliferation by CCK assay in the control and RGS17 knockdown cells. Data represent the mean ± SD from triplicate samples and were representative of three independent experiments. *p<0.05, **p<0.01. (D) Effects of RGS17 on the cell migration and invasion by Boyden chamber assay. The mean ± SD shows the number of migrated and invaded cells obtained from three parallel experiments. **p<0.01, ***p<0.001.