Figure 7.

Effects of Huaier extract on expression of MAPK in HCC cells. HCC cells were treated with various concentrations (0, 2, 4, 8, and 16 mg/mL) of Huaier extract for 48 h, and then cell lysates were subjected to Western blotting using respective antibodies against MAPKs. Total ERK1/2, p38, JNK, and GAPDH were included as the loading controls, and the results are expressed as the mean densitometric ratios ± SD in three independent experiments. ^∗ P < 0.05, ^∗∗ P < 0.01, and ^∗∗∗ P < 0.001, compared with that of the untreated control, respectively (0 mg/mL).