Figure 8.

Effect of Huaier extract on the expression of β-catenin and cyclin D1, as well as cell cycle and inhibition after being treated with a JNK inhibitor (SP600125). (a) HepG2 cells were pretreated with SP600125 (10 μM) for 1 h and then incubated in the presence or absence of Huaier extract (8 mg/mL) for 48 h. β-catenin and cyclin D1 protein was determined by using Western blotting. (b) Cells were also assessed by cell cycle. Data are presented as the means ± SD of at least three independent experiments. ^∗∗ P < 0.01, ^∗∗∗ P < 0.001, untreated cells versus SP600125 or Huaier extract; ^# P < 0.05, ^### P < 0.001 Huaier extract versus SP600125 plus Huaier extract.