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. Author manuscript; available in PMC: 2015 Jul 16.
Published in final edited form as: Nat Struct Mol Biol. 2011 Aug 14;18(9):971–976. doi: 10.1038/nsmb.2099

Figure 2.

Figure 2

Identification of phosphorylated residues in JAK2 JH2. (a) Chromatogram of JAK2 JH2 purification showing the peaks from anion-exchange chromatography. (b) Coomassie staining of a native-gel electrophoresis of JH2-Peak1 and JH2-Peak2 proteins. (c) Coomassie staining of a native-gel electrophoresis of purified JH2-Peak1 and JH2- Peak2 after kinase reaction. (d) MS-MS spectra of the phosphorylated residues in JAK2 JH2-Peak2 4h kinase assay. Left: JH2-Peak2 is stoichiometrically phosphorylated at Ser523. Right: JH2-Peak2 is partially phosphorylated at Tyr570.