Fig. 6.

Assessment of extrinsic- and mitochondrial-pathways in apoptosis induced by CDKs inhibitors in KCL22 cells. a KCL22 cells were treated with 10 μM of drugs for 24 h before western-blotting analysis of initiator and effector caspases and lamin A/C. b Kinetics of apoptotic process induced by Roscovitine-derived CDKs inhibitors. Time-dependent cleavage of caspases revealed by western-blotting analysis of KCL22 cells treated by 10 μM R-CR8 for indicated times. Actin was used as a sample loading control. c Analysis of KCL22 Annexin V-positive cells pretreated with 100 μM of caspase inhibitors then treated for 24 h with 10 μM R-CR8. Results are expressed as apoptotic cells fold increase versus negative untreated cells. Experiment was done twice