Figure 5. Tetanus Toxin Expression in Proprioceptors Blocks Sensory-Motor Transmission.

(A and B) Extracellular recordings from ventral root L5 following dorsal root L5 stimulation in p8 wild-type and Pv^TeNT mice. Trace averaged across 5 trials at 0.1 Hz. Black arrow indicates stimulus artifact. Traces are shown in a time expanded scale in (B).

(C) Reflex amplitude is reduced by 92% in p8 Pv^TeNT mice. Differences are significant at p < 0.001 (Student’s t-test, n = 3 mice).

(D and E) Intracellular recordings from L4 motor neurons following dorsal root L4 stimulation in p4 wild-type and Pv^TeNT mice. Single trials shown. Traces are shown in a time expanded scale in (E). First dashed line indicates onset of EPSP response. Second dashed line indicates the maximum amplitude of the monosynaptic response, as determined at 3 ms after EPSP onset.

(F) Monosynaptic EPSP amplitude is reduced by 96% in p4 Pv^TeNT mice. Differences are significant at p < 0.001 (Student’s t-test; n = 4 MNs).

All data reported as mean ± SEM. For related data, see also Figure S5.