Figure 3.

Effect of PARP inhibition on PDZK1 mRNA in E₂-stimulated MCF-7 cells. MCF-7 cells were treated with E₂ for 24 h in the absence or presence of 1 or 2 μM TIQ-A. Total RNA was prepared and subjected to reverse transcription followed by conventional (a) or quantitative (b) PCR with primers specific to human PDZK1 or β-actin. ^¶Difference from untreated controls; ^§difference from E₂-treated cells; p < 0.05. c MCF-7 cells were transduced with a viral vector encoding control shRNA or a shRNA targeting human PARP-1. Cells were then treated with E₂ for 24 h after which total RNA was prepared and subjected to conventional RT-PCR with primers specific to human PDZK1 or β-actin.