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. 2015 Jul 17;5:38. doi: 10.1186/s13578-015-0033-y

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© Chou et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The basal expression levels of AR, cPAcP and PSA were determined in different PCa cell lines. LNCaP C-33/C-81, LNCaP C4-2/C4-2B, MDA PCa2B AS/AI, PC-3 and DU 145 cells that were plated in regular medium for 3 days and then changed with fresh medium for 1 day. The cells were harvested and the total protein was subjected to western blot analyses of functional proteins expression. β-Actin was analyzed and used as a loading control.