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. 2015 Jul 17;5:38. doi: 10.1186/s13578-015-0033-y

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© Chou et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of VPA on androgen responsiveness of PCa cell lines. a LNCaP C-33; b LNCaP C4-2B; c MDA PCa2B AS cells were seeded in 6-wells plate and then treated with 1 mM VPA or solvent for 48 h. Cell were then maintained in a steroid-reduced medium with or without 10 nM DHT for 2 days. Total cell number was counted. The ratio of cell growth was calculated by normalizing the cell number to that of control cells (column #1, left panel, n = 3×2). Total cell lysate proteins from 3-day DHT treatment were analyzed for cPAcP, PSA, AR protein. β-Actin was analyzed and used as a loading control (right panel).