Fig. 4.

Elevating [Mg²⁺]_i leads to increased Ca²⁺-sensitivity-related proteins in terminals. (a) Schematic cartoon illustrates the three critical biophysical factors affecting presynaptic vesicle release. (b and c) Immunofluorescence. No change of quantity of Synapsin1, Ca_v2.1 and Ca_v2.1 (b), but remarkable increase in Ca²⁺-sensitivity-related proteins at dendritic branches (c) after elevating [Mg²⁺]_o for 4 hr. Pseudo-color scale: fluorescent intensity. (d) Elevating [Mg²⁺]_o for 4 hr led to increase in Ca²⁺-sensitivity-related protein positive fluorescence in terminals at dendritic branches (n = 10–21 coverslips for each point). The mean ± SEM of coverslips was presented. Two-tailed Student’s t-test, comparing 1.2 4hr to Ctrl 0.8, n.s. no significance, *** p < 0.001