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. 2015 Jul 16;10(7):e0133012. doi: 10.1371/journal.pone.0133012

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© 2015 Roy et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — a) UV-Vis spectra of HSA in presence of 4PBA. Cuvette concentration, cHSA: 1μM (a) and c4PBA (1,2,4,8,12,16,20 μM): b→h; pH 7.4 at 25°C. Inset shows slight blue shift at the Tryptophan absorption region indicated by the arrow. b) Effect of ethanol on the fluorescence emission intensity of HSA. cHSA = 1μM, cEthanol =, 1,2,4,8,12,16,20 μM (pH 7.4, 25°C) c) Fluorescence emission spectra of HSA in presence of 4PBA. cHSA: 1μM (a) and c4PBA (2,4,8,12,16,20 μM): b→g; pH 7.4 at 25°C.Dotted line represents contribution of4PBA (20μM) at the emission range of HSA. d) Plot of 1/F-F₀ vs 1/ [4PBA]